The timing of wound in forensic medicine is one of. the cardinal problems..
For the practical determination of skin injuries, serveral authors have pointed out the importance of histopathological, histochemical, ultrastructural studies and the study by fluorescent antibody. technique.
In addition to the above method, the above authors found that the application of peroxidase-labeled antibody technique to a medicolegal study on timing of wounds had resulted in an improved understanding of the estimation of the lapse of time in wound.
Rats of both sexes weighing about 200 gm were used and linear skin wounds were cut in the shaved dorsal area.
After the infliction of wounds, skin flaps of 1 x lcm were removed 1,2,3,4, 5, 8,. 12 and 24 hours, and 2,3,4,5 and 7 days for examination.
One of the flaps was fixed in formalin solution for hematoxylin-eosin stain and the other was performed the frozen section for the application of immunoperoxidase technique by the light and electron microscopic study.
The results were as follows:
1. In the vicinity of a 1-hour-old wound, polymorphonuclear leukocyte infiltration was seen. After 12 hours, macrophages appeared larger in number than polymorphonuclear leukocytes.
2. At 1 hour, the frbrin in wound formed a fine delicate meshwork, and then it became coarser fibrils in layers and a gradually concentrating network by the
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